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Flat-mounted retina from P13 mouse (after 75% O2 from P7 - P12) stained with anti collagen type IV (vessels in green) and anti EF5 (red, hypoxic areas). The animal was injected with the drug EF5 two hours before sacrifice. In hypoxic regions, the drug is reduced and forms permanent protein adducts that can be recognized with an antibody. (Unpublished, courtesy of Marcus Fruttiger).
