Table 2.

Interactions of oestrogen status and AnxA1 on LPS-induced cell flux in cerebral venules

	Cell flux (cells·min−1)
	WT (intact)		WT			AnxA1−/−
	Male	Female	Sham	OVX	E2	Sham	OVX	E2
Saline	0.2 ± 0.2	0.3 ± 0.3	2.0 ± 1.2	5.5 ± 1.0b	4.8 ± 0.8b	2.5 ± 2.5	11.3 ± 4.2b	5.0 ± 2.4c
LPS	2.3 ± 0.8a	2.7 ± 0.7a	13.8 ± 6.3a	28.8 ± 5.5a,b	12.0 ± 1.6a,c	16.5 ± 5.7a	25.5 ± 2.1a	18.3 ± 1.2a

The following mice were used: male and pro-oestrous female C57BL/6 (termed WT intact); female C57BL/6 (WT) and AnxA1^−/− mice, some of which were ovariectomized and treated daily for 8 d with 40 ng 17β-oestradiol per mouse; s.c. (termed E2) or vehicle (termed OVX); controls were subjected to sham operation only (termed sham). All groups were injected with LPS (10 μg per mouse) or saline vehicle (100 μL) i.p., and after 2 h, the leukocyte–endothelial cell interactions in cerebral venules were quantified by intravital microscopy in terms of rolling leukocyte flux (expressed as cells·min⁻¹). Data are expressed as mean ± SEM. n = 4–6 mice per group.

^aP < 0.05 versus saline vehicle-treated counterparts.

^bP < 0.05 versus sham counterparts.

^cP < 0.05 versus OVX counterparts using anova followed by Bonferroni's post hoc test.