. 2013 May 16;169(3):539–553. doi: 10.1111/j.1476-5381.2012.02146.x

Table 3.

LPS-induced changes in plasma cytokine concentrations in ovariectomized female WT and AnxA1^−/− mice

		[TNF-α] (pg·mL⁻¹)		[IL-6] (pg·mL⁻¹)		[IL-10] (pg·mL⁻¹)
		Vehicle	LPS	Vehicle	LPS	Vehicle	LPS
WT	Sham	0	2177.0 ± 853.1^a	0	4259.0 ± 72.4^a	0	1004.0 ± 501.9^a
	OVX	0	220.6 ± 128.5^a^,^b	0	2086.0 ± 254.2^a^,^b	0	0^b
	E2	0	176.3 ± 54.2^a^,^b	0	2022.0 ± 170.3^a^,^b	352.2 ± 130.7^b^,^c	47.9 ± 16.7^a^,^b
AnxA1^−/−	Sham	0	4542.0 ± 2477.0^a	0	4230.0 ± 47.2^a	0	1232.0 ± 306.7^a
	OVX	0	0^b^,^d	0	1854.0 ± 272.8^a^,^b	0	58.3 ± 13.8^b
	E2	34.6 ± 3.4	205.5 ± 53.1^a^,^b^,^c	0	2215.0 ± 70.7^a^,^b	35.8 ± 5.51^d	76.9 ± 23.7^b

Saline vehicle (100 μL) or LPS (10 μg per mouse) was administered i.p. to ovariectomized C57BL/6 (WT) and AnxA1^−/− mice receiving daily oestrogen replacement therapy (E2) or peanut oil vehicle (OVX). Blood was taken 2 h later by cardiac puncture and plasma assayed by elisa for TNF-α, IL-6 and IL-10. A value of 0 indicates that cytokine concentrations were below the detectable limits of the kits (31.25 pg·mL⁻¹ for TNF-α and IL-10; 15.63 pg·mL⁻¹ for IL-6). Data are mean ± SEM. n = 3–4 mice per group.

P < 0.05 versus vehicle-treated counterparts.

P < 0.05 versus sham-operated counterparts.

P < 0.05 versus OVX counterparts.

P < 0.05 versus WT counterparts using anova with Bonferroni's post hoc test.