Figure 2.

Agonist potency and efficacy of RO5527239 in comparison to LCA in recombinant CHO cells. (A) Intracellular cyclic AMP levels in the human (full symbols) and mouse (open symbols) CHO GPBAR1-expressing stable cell lines treated for 30 min with RO5527239 (triangle) and LCA (circle). Data are the means from three independent experiments, each of which is the mean of three samples. (B) Gene-dosing experiments with human (full symbols) and mouse (open symbols) GPBAR1 receptors in transiently transfected CHO cells and treated for 30 min with 1 μmol·L⁻¹ RO5527239 (triangle) and 10 μmol·L⁻¹ LCA (circle) or buffer (diamonds). Data represent mean cAMP levels ± SEM of three independent experiments made in triplicate.