Figure 3.

Agonist potency and efficacy of RO5527239 in comparison to DCA in STC-1 cells. (A) Effect of GPBAR1 agonists RO5527239 and the BA DCA on intracellular cAMP accumulation. STC-1 cells were stimulated with the indicated reagents and incubated for 15 min at 37°C in the presence of IBMX (1 mmol·L⁻¹). (B) GPBAR1 activation promotes GLP-1 secretion from STC-1 cells in response to RO5527239 along with the BA DCA. STC-1 cells were incubated for 2 h at 37°C in Krebs-Ringer buffer containing various concentrations of RO5527239 and DCA. After incubation, the conditioned medium was collected and the concentration of GLP-1 was determined by enzyme immunoassay. Data represent the mean ± SEM of three independent experiments made in triplicate.