Figure 3.

Identification of cis-regulatory elements using the α-globin promoters as bait, and analysing 4C material by HTS (a) The terminal 500 kb region of chromosome 16 (HS18 16p13.3 as described in figure 2a). (b) Comparison of analysis by microarray (erythroid 1 array, signal on the y-axis is normalized for the total signal on the array) and peHTS of 4C inverse amplifications from the α-globin promoters (indicated by red asterisks). Two biological replicates of erythroid 4C libraries were analysed (erythroid 1 peHTS and erythroid 2 peHTS). The peHTS data were treated as single-end reads that were then ‘binned’ into DpnII restriction fragments (see text). (c) Shows the same data as displayed in the lower panel of (b) for erythroid 2 peHTS analysed using the sequences' paired-end relationships to identify junction fragments (one of which must map to the α-globin genes; chr16:158 550–169 250) and displayed using a custom looping glyph (promoter fragments shown as red asterisks).