Table 1.
Primer sequences and annealing temperatures.
| target | name | sequence | position | annealing temperature (°C) |
|---|---|---|---|---|
| HBA promoter | HBA3Cf | TTCCCCACCACCAAGACCTAC | chr16:163 137–163 157 | 66 |
| chr16:166 941–166 961 | ||||
| HBA3Cr | AGAAAGTCAGCCCGCACCCC | chr16:162 497–162 516 | ||
| chr16:166 301–166 320 | ||||
| MCS-R2 (HS-40) | HS40f | CTGCTGATTACAACCTCTGGTGC | chr16:103 705–103 727 | 58 |
| HS40r | GAGCCTGGGGGAAAGGAGTAG | chr16:102 893–102 913 | ||
| HBB promoter | HBBPf1 | TGAGGTCTAAGTGATGACAGCCG | chr11:5 204 981–5 205 003 | 57 |
| HBBPr1 | TGAGGAGAAGTCTGCCGTTACTGC | chr11:5 204 787–5 204 810 | ||
| axin CTCF site | AXINf1 | GATGAGCAGAATCTGGTGATGAACAG | chr11:352 622–352 647 | 58 |
| AXINr1 | GAAGAGACAAAGGGAGCAGGGTG | chr11:351 223–351 245 |