Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Commun. 2013;4:1966. doi: 10.1038/ncomms2966

Figure 2. Forced expression of Zscan4 enhances developmental potency of ES cells.

Figure 2

a, Schematic presentation of experimental procedure. b, Expression levels of Zscan4 (endogenous and ORF) in Dox+ and Dox- conditions in G418-selected clones. Error bars indicate S.E.M., n=3 c, Forced expression of βgeo in Dox+ and Dox- conditions. d, Induction of Stra8 and Tmem92 (Zscan4-downstream genes) in Dox+ condition. Error bars indicate S.E.M., n=3 e, Forced expression of Zscan4 protein detected by immunohistochemistry. Scale bar, 100 μm. f, Assessment of developmental potency of ES cells by 4N complementation assays.