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Tetraploid (4N) complementation assays confirm the higher potency of ES cells expressing a Zscan4c-ERT2 fusion protein. Single ES cells were injected into 4N blastocysts.
| Cell line | No. Injected cells | Passage # | No. blastocysts transferred | No. embryos at full term (%) | No. live embryos (%) |
|---|---|---|---|---|---|
| V6.5 | 1 | 18 | 71 | 1(1) | 1(1) |
| V6.5 #2 | 1 | 21 | 77 | 0(0) | 0(0) |
| V6.5 ZERT2 #18 | 1 | 21 | 44 | 3(7) | 2(5) |
| TA1 | 1 | 4 | 79 | 3(4) | 3(4) |
