FIG. 5.

Schematic overview of the optimal protocol for counting of viruses in solution. Superscript letters: a, 0.5% final concentration; b, store sample at temperatures below −80°C; c, work fast once samples are thawed since total virus count decreases with time; d, TE buffer at pH 8 and avoid low dilution factors (<10); e, final dilution SYBR Green I of 5 × 10⁻⁵ the commercial stock; f, allow sample to cool down for 5 min in the dark before analysis.