TABLE 3.
Statistical ANOVA of different treatments tested in detail for phytoplankton viruses and heterotrophic bacteriophages together (6 to 10 viruses tested per treatment)a
| Expt | Factor treatment | Levels | Log
|
||
|---|---|---|---|---|---|
| Counts | GFL | SSC | |||
| Incubation | Temp | 5 | ** | ||
| Incubation | Time | 7 | * | ||
| Fixation | Glutaraldehyde | 4 | |||
| Fixation | Formaldehyde | 4 | * | ||
| SYBR Green 1 | Dye concn | 6 | * | ||
| SYBR dyes | Gold vs Green I | 2 (yes/no) | * | ||
| SYBR Gold | Dye concn | 3 | * | ||
| Dilution | Solution | 5 | * | ||
| TE solution | pH | 6 | |||
| Dilution factor | Addition of seawater | 7 | * | * | |
| Additives | Detergents | 5 | ** | ||
| Additives | Citrate | 7 | |||
| Storage | T = 1 h | 4 | * | ||
| Storage | T = 1 mo | 4 | *** | * | |
| Storage | 4°C | 4 | ** | ** | |
a
A two-way ANOVA with viruses as a random factor (block factor) and a treatment on two to seven levels was used. The treatments that were found to significantly affect total viral counts, GFL, or SSC of the stained virus particles are indicated by asterisks: *, P < 0.05; **, P < 0.01; and ***, P < 0.001. Levels are as described in Materials and Methods.