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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Mol Microbiol. 2010 May 4;77(1):44–55. doi: 10.1111/j.1365-2958.2010.07194.x

Figure 4.

Figure 4

Oxygen consumption profiles for V. fischeri strains demonstrating the response to treatment with 80 µM DEA NONOate. Cells were grown in mineral salts medium containing GlcNAc to mid-log phase and placed in the respirometer chamber. DEA NONOate was injected into the chamber at an oxygen concentration of 190 µmol/L (indicated by the arrow). A. Profiles for wild type (solid line; OD600 of 0.310, total protein content of 1.29 mg) and the ΔnsrR strain AKD711 (dashed line; OD600 of 0.304, total protein content of 1.28 mg). The oxygen consumption profiles for AKD780 (aox mutant) and AKD786 (aox nsrR double mutant) were not consistently different from those of the wild type and nsrR mutant, respectively (data not shown). B. Profiles for AKD788 (expressing only CydAB; solid line; OD600 of 0.299, total protein content of 1.25 mg) and AKD789 (expressing only AOX; dashed line; OD600 of 0.311, total protein content of 1.27 mg). Graphs demonstrate oxygen consumption profiles for one set of cultures and are representative of three independent experiments.