Table 1.
Influence of nitric oxide (NO) on aoxtranscript levels and aox promoter activity in V. fischeri
| aox relative transcript levels or promoter activity | |||
|---|---|---|---|
| assay | −NO | +NO | fold-induction |
| qRT-PCR* | |||
| (ES114) | 0.03(.004)** | 1.23(0.14) | 41 |
| β-galactosidase assay*** | |||
| plasmid reporter | |||
| (ES114 pAKD500) | 15(2.2) | 65(22) | 4.3 |
| chromosomal reporter | |||
| (AKD712) | 2.9(0.2) | 86(20) | 30 |
| chromosomal reporter | |||
| (ΔnsrR, AKD785) | 876(88.1) | 735(23.2) | 0.8 |
*
data presented were calculated using the comparative CT method using the reference gene rpoD
**
number in parentheses indicates standard error of the mean
***
β-galactosidase activity is reported in Miller units