Table 1.
New SSR loci developed for Paspalum
| Locus name | Repeat motif | Ta(°C) | Product size(bp) | NM | PIC | F(5´-3´) | R(5´-3´) | GenBank accession number |
|---|---|---|---|---|---|---|---|---|
| PN02-B3 |
(GT)12 |
50 |
139-157 |
11 |
0,8 |
CAAACAATGGGACAACACTT |
TTTTTCTGCTCTGGCTCAT |
HQ585421 |
| PN02-C6 |
(TG)8 |
51,4 |
139-145 |
4 |
0,3 |
TTCAGCAATCAAGAGTTAGA |
AGAGACGATCAGGGTGTG |
HQ585422 |
| PN02-G3 |
(GT)9 |
60 |
294-302 |
5 |
0,6 |
GTCGCCGGTCGCAGTCG |
CACATAGCCGGCCTCCTCTC |
HQ585423 |
| PN02-G7 |
(AC)7 |
TOUCHDOWN |
118 |
7 |
0,7 |
TGCAGCATGTAATAACC |
TACACAGGAAGGAAGAAA |
HQ585424 |
| PN03-C7 |
(GT)6. |
50 |
159-165 |
3 |
0,6 |
TCATGCCACGAGAAAGAAACCA |
CAGGGACGACAGGGACAAGACT |
HQ585425 |
| PN03-D10 |
(GT)11 |
50 |
280-300 |
7 |
0,7 |
GTTATTCCTTCACTCACTCACC |
AGCTTTCTGCCTTCTTTTT |
HQ585426 |
| PN03-D12 |
(AG)10 |
50 |
220-240 |
7 |
0,7 |
TGGGGAAGCAGGAAGTCACA |
CAGCTCACCGATGGGAATG |
HQ585427 |
| PN03-E7 |
(GT)9 |
TOUCHDOWN |
298 |
7 |
0,7 |
CATGCTTTTCTGCTTCC |
GCTAATACTGCTTGCTCTTC |
HQ585428 |
| PN03-F1 |
(CA)7 |
50 |
184-192 |
5 |
0,7 |
CAGTGACCAAGCTTACAACCTA |
GTGGCATGCCTTTCTACAA |
HQ585429 |
| PN03-F10 |
(TG)7 |
60 |
165-177 |
7 |
0,8 |
TGCCTACCGTTTCCTCTTCTCT |
GGGATGGGTCTTGACTCTTGAT |
HQ585433 |
| PN03-F3 |
(GT)8 |
60 |
165-203 |
8 |
0,8 |
ACCCCGCGTCCTCTTTCTCA |
CCCAACCCACCATTATCCTCTG |
HQ585430 |
| PN03-F7 |
(CA)10 |
50 |
200-212 |
6 |
0,8 |
TGCTGGCAGTCTTTCTCT |
GGCCATACCACTTTTTG |
HQ585431 |
| PN03-F9 |
(CA)8 |
60 |
179-275 |
15 |
0,9 |
AAAAGGGCAGGAGTTAGGAC |
AGTTGGCTCAGTTACGATTTTA |
HQ585432 |
| PN03-G5 |
(CA)7 |
60 |
126-138 |
6 |
0,7 |
AAGTCACCGTTTCAATCCAG |
GCAGTGCGCAAGTTTTCT |
HQ585434 |
| PN03-H3 |
(GA)22 |
51,4 |
198-250 |
11 |
0,8 |
CATGTAAACGTAGCAGAGGT |
GCTAATTTTACAGGGTGGTC |
HQ585435 |
| PA01-C8 |
(AT)2TTA(AT)2TTCA(GA)2 |
60 |
247-279 |
6 |
0,8 |
AATCTGACCTGTTTTACTTCTC |
TGCATTTTTGGGATACACT |
HM208140 |
| PA01-E10 |
(GGT)2 T(ACT)2 |
54 |
186-210 |
6 |
0,4 |
GCGATTATTGTTGTTTGGTTTG |
ACGCTTTCTTGTCTTCATCTCA |
HM208141 |
| Average | 7,1 | 0,7 |
Ta primer annealing temperature, TD touchdown, NM number of markers, PIC polymorphism information content.
PN Paspalum notatum SSR loci, PA P. atratum SSR loci.