Table 7.
Effect of aqueous extract of Terminalia paniculata on apoptotic markers in CCl4-treated Chang liver cells
| Groups | Phospho-p53 | p53 | Cleaved caspase-3 | Phospho-Bad | Bad | Cleaved PARP |
|---|---|---|---|---|---|---|
|
Normal control |
0.134 ± 0.037 |
0.129 ± 0.020 |
0.126 ± 0.023 |
0.141 ± 0.024 |
0.146 ± 0.040 |
0.135 ± 0.042 |
|
TPW (50 μg/ml) control |
0.130 ± 0.025 |
0.131 ± 0.028 |
0.123 ± 0.020 |
0.136 ± 0.033 |
0.152 ± 0.059 |
0.138 ± 0.066 |
|
CCl4control |
1.896 ± 0.081* |
2.069 ± 0.054* |
2.182 ± 0.091* |
1.758 ± 0.037* |
2.367 ± 0.072* |
2.211 ± 0.101* |
|
TPW (25 μg/ml) + CCl4 |
1.384 ± 0.066*,# |
1.514 ± 0.033*,# |
1.487 ± 0.040*,# |
1.324 ± 0.051*,# |
1.868 ± 0.064*,# |
1.445 ± 0.047*,# |
| TPW (50 μg/ml) + CCl4 | 1.193 ± 0.050*,# | 1.148 ± 0.057*,# | 1.075 ± 0.072*,# | 1.219 ± 0.035*,# | 1.644 ± 0.049*,# | 1.117 ± 0.053*,# |
All values are expressed as mean ± SEM and experiments were carried out in triplicate. *p < 0.05 vs. normal control; #p < 0.05 vs. CCl4 control. The levels of the apoptotic markers were expressed in terms of absorbance as per kit’s protocol.