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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: JACC Cardiovasc Imaging. 2012 Dec;5(12):1253–1262. doi: 10.1016/j.jcmg.2012.05.017

Figure 4. Targeted Transfection of Luciferase Reporter Plasmid.

Figure 4

(A) In vivo optical imaging data of luciferase reporter gene transfection 3 days after CUMGD, quantified as photon flux at the site of ultrasound exposure 10 min after intraperitoneal injection of luciferin. PNAP = peak negative acoustic pressure. Transfection was significantly lower at 0.6 MPa compared with 1.0 and 1.8 MPa for both agents. (B) Examples illustrating luminescence (color-coded) 3 days after bilateral hindlimb ischemia and intravenous injection of either nontargeted or P-selectin–targeted cDNA-coupled cationic microbubbles during ultrasound at 0.6 MPa. Ventral surface depiliation was performed to reduce light attenuation. (C) Immunohistochemistry for luciferase with peroxidase illustrating transfection (arrows) of venular and capillary endothelium and perivascular cells. Scale bar = 50 μm.