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. 2013 Jun 14;8(6):e66719. doi: 10.1371/journal.pone.0066719

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© 2013 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Purified recombinant HA proteins were placed on SDS-PAGE gels and deglycosylated using PNGase F. Glycans were isolated from protein backbones and analyzed using HILIC-HPLC to identify individual glycan structures. A dextran ladder was created with HILIC-HPLC and used as a standard to provide glucose unit (GU) values for individual peaks recognized in glycan samples from recombinant HA proteins. Shown are HA N-glycan profiles of (A) Sf9-rHA, (B) Mimic-rHA, and (C) CHO-rHA. Red inverted triangles, fucose; blue squares, N -acetylglucosamine; green circles, mannose; yellow circles, galactose; pink diamonds, sialic acid.