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. 2013 Jun 14;8(6):e66719. doi: 10.1371/journal.pone.0066719

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© 2013 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The antibody isotypes of IgM and IgA and the IgG1, IgG2a, IgG2b and IgG3 subtypes in sera were analyzed using ELISA plates coated with (A) Sf9-rHA or (B) CHO-rHA. Mouse splenocytes were collected 3 wk following second inoculations, and then reacted with (C) Sf9-rHA or (D) CHO-rHA to measure the numbers of antibody-secreting B cells in spleens; ELISPOT assays were performed using HRP-conjugated anti-mouse IgG antibodies. Data represent mean ± standard deviation; results were analyzed using one-way ANOVAs with Tukey's tests, with statistical significance at p<0.05 as asterisks indicated.