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The impact of antisense RNA expression on the in vivo accumulation of HindIII-digested phage κ3-specific DNA fragments over time. (A) Vector control strain NCK1125(pTRK687); (B) NCK1125(pTRK789::pri3.8-AS); (C) NCK1125(pTRK790::pri3.10-AS). Each plasmid encoded two HindIII sites; plasmid-specific DNA bands are demarked with black triangles. Abbreviations: φ, purified phage κ3 genomic DNA; M, 1-kb molecular size marker (GIBCO-BRL).
