TABLE 2.
Primers used in this study
| Primera | Nucleic acid sequenceb | Positionc |
|---|---|---|
| S1 | 5′-AAACTGCAGCAGAGAACAATTTGCAAGC-3′ | 28,575 |
| S2 | 5′-AAACTGCAGCAACACCCAAGAGCC-3′ | 28,466 |
| S3 | 5′-AAACTGCAGTAAGGAGGATTGGACTTGAC-3′ | 28,534 |
| S4 | 5′-AAACTGCAGTTGACAACGATTGATTTCG-3′ | 28,549 |
| S5 | 5′-AAACTGCAGATTAATTTTAGTACCATTG-3′ | 29,053 |
| S6 | 5′-AAACTGCAGGGTACATATCGACGTATCG-3′ | 29,557 |
| S7 | 5′-AAACTGCAGTAGCTATATATGATCCAG-3′ | 28,787 |
| S8 | 5′-CCCAAGAGCCTTTGGGCAATAAGG-3′ | 28,471 |
| A1 | 5′-AAACTGCAGGTTGCAATAACCTGCGG-3′ | 30,080 |
| A2 | 5′-AAACTGCAGGTGAGTAACCATAACCAC-3′ | 30,060 |
| A3 | 5′-AAACTGCAGAAACTTATGGTCAAACGATAG-3′ | 29,052 |
| A4 | 5′-AAACTGCAGGTTTGACTTATTCTTAAACAC-3′ | 29,556 |
| A5 | 5′-AAACTGCAGCTTTCCCATTTTCGAGGG-3′ | 28,786 |
| A6 | 5′-CTAAGTAACTAAAGCAACCGAACCC-3′ | 30,135 |
| P6 | 5′-GGAGCGTGATTTTTATGG-3′ | —d |
| T7 | 5′-GCTTCCGGCTCGTATGTTGTGTGG-3′ | — |
a
S and A primers are derived from the sense and antisense strands, respectively.
b
PstI (5′-CTGCAG-3′) restriction sites are underlined when appropriate.
c
The 5′ nucleotide position relative to the DT1 sequence is shown in bold when appropriate.
d
—, not applicable.