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. 2013 Jun 14;8(6):e66483. doi: 10.1371/journal.pone.0066483

Table 1. Composition of each type of medium applied in this study.

Component (mg/L)	Modified Chu13	1/4 ASWM	1/4 NSWM	NaCl medium
KNO₃	600	600^a	600	600
MgSO₄⋅7H₂O	100			100
CaCl₂⋅2H₂O	54	331.5		54
K₂HPO₄⋅3H₂O	52	52^a	52	52
FeNaEDTA	10	10^a	10	10
H₃BO₃	2.36	2.36^a	2.36	2.36
MnSO₄⋅H₂O	1.54	1.54^a	1.54	1.54
ZnSO₄⋅7H₂O	0.22	0.22^a	0.22	0.22
CoSO₄⋅7H₂O	0.09	0.09^a	0.09	0.09
CuSO₄⋅7H₂O	0.08	0.08^a	0.08	0.08
Na₂MoO₄⋅2H₂O	0.06	0.06^a	0.06	0.06
NaCl		5186.75		8800
MgCl₂⋅6H₂O		2368.5
Na₂SO4		876.25
KCl		149.25
NaHCO₃		42.75
KBr		21.25
Na₂B₄O₇⋅10H₂O		8.5
SrCl₂		3
NaF		0.75
LiCl		0.25
KI		0.0175
(NH₄)₆Mo₇O₂₄⋅3H₂O		0.005
AlCl₃⋅6H₂O		0.002
FeCl₃⋅6H₂O		0.00125
MnCl₂⋅4H₂O		0.0002
Na₂WO₄⋅2H₂O		0.00005
Natural seawater			250 ml^b
Deionized water	1000 ml	1000 ml	750 ml	1000 ml

^a

Added to offset differences compared with the modified Chu13 medium.

^b

Two hundred and fifty milliliters of natural seawater taken directly from the open sea.