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. 2013 Jun 14;8(6):e65301. doi: 10.1371/journal.pone.0065301

Table 1. Flooding-responsive proteins identified in soybean root using LC-MS/MS.

Theoretical
Spot no Homologous protein Species Protein ID Accession no Mr (Da) pI Score MP Cov(%) BlastScore
1 Kunitz trypsin protease inhibitor [Glycine max] Glyma09g28310.1 ACA23207 22947 5.24 28 2 15 397
2 predicted protein [Populus trichocarpa] Glyma03g34820.1 XP_002327377 20260 6.13 26 2 15 212
3 (No significant hits to report)
4 enolase [Glycine max] Glyma03g34830.1 AAS18240 47914 5.49 72 2 13 863
5 Heat shock 70 kDa protein [Glycine max] Glyma17g08020.1 P26413 71220 5.28 2152 25 38 1144
6 U2 small nuclear ribonucleoprotein A [Ricinus communis] Glyma20g18440.1 XP_002512712 32537 5.16 104 3 18 405
7 UDP-glucose 6-dehydrogenase [Glycine max] Glyma08g26520.1 Q96558 53478 5.74 66 2 14 936
8 dihydroxyacid dehydratase [Glycine max] Glyma13g27810.1 ACU26534 64644 5.76 314 23 25 1160
9 V- ATPase subnit A [Glycine max] Glyma08g23990.1 ABU87506 69020 5.48 281 47 26 1217
10 predicted protein [Populus trichocarpa] Glyma09g40690.1 XP_002323696 61059 5.51 1049 104 30 949
11 oligopeptidase A [Medicago truncatula] Glyma01g02480.1 ABY48141 88745 6.03 777 84 43 1227
12 Proteasome subnit alpha type-6 [Glycine max] Glyma06g39710.1 O48551 27481 5.58 1406 107 47 489
13 glutathione reductase [Vigna unguiculata] Glyma16g27210.1 ABB89042 54243 5.63 629 79 48 880
14 hypothetical protein [Vitis vinifera] Glyma06g48360.1 XP_002272730 67578 5.60 765 89 39 1075
15 isoflavonoid malonyl transferase 2 [Medicago truncatula] Glyma18g49240.1 ABY91222 56964 5.70 601 75 29 430
16 predicted protein [Populus trichocarpa] Glyma05g37670.1 XP_002298026 112704 5.89 69 9 15 1470
17 hypothetical protein [Vitis vinifera] Glyma18g04730.1 XP_002270157 82072 6.21 225 15 17 1216
18 UDP-D-apiose/xylose synthetase [Gossypium hirsutum] Glyma11g19550.1 ACJ11753 43680 5.83 283 47 27 728
19 hypothetical protein [Vitis vinifera] Glyma02g36530.1 XP_002276114 60039 6.69 70 7 16 854
20 isocitrate dehydrogenase [NADP] [Glycine max] Glyma02g40820.1 Q06197 46393 5.87 1474 124 40 828
21 UDP-glucose 6-dehydrogenase [Glycine max] Glyma08g26520.1 Q96558 53478 5.74 141 8 19 936
22 seed maturation protein PM31 [Glycine max] Glyma02g08400.1 AAD30865 17907 6.10 57 2 19 323
23 elongation factor 1-gamma [Glycine max] Glyma16g00360.1 AAL82617 47785 5.92 117 2 15 619
24 nucleoside diphosphate kinase [Glycine max] Glyma03g25820.1 AAN77501 16308 6.30 76 2 12 284
25 cysteine proteinase inhibitor [Glycine max] Glyma13g25870.1 BAA19608 27708 6.57 57 5 10 417
26 Transaminase mtnE [Ricinus communis] Glyma08g06790.1 XP_002522052 50463 6.67 607 54 32 721
27 acetoacetyl-CoA thiolase [Medicago sativa] Glyma14g00760.1 ACX47470 41930 6.16 469 46 39 678
28 hypothetical protein isoform 2 [Vitis vinifera] Glyma01g06970.1 XP_002269733 53522 6.34 448 44 38 890
29 acetoacetyl-CoA thiolase [Medicago sativa] Glyma14g00760.1 ACX47470 41930 6.16 62 2 13 678
30 Gamma-glutamyl hydrolase [Glycine max] Glyma13g34290.1 P93164 38282 6.72 3208 212 36 619
31 serine hydroxymethyltransferase 2 [Glycine max] Glyma05g28490. ACM45952 52142 6.90 982 67 25 920
32 heat shock protein 70 [Gossypium hirsutum] Glyma19g35560.1 ACJ11741 71854 5.05 3704 251 37 1135
33 actin isoform PEAc14-1 [Pisum sativum] Glyma02g10170.1 ADP09679 42036 5.23 682 107 51 754
34 glutamate decarboxylase [Glycine max] Glyma18g04940.1 BAF80896 57554 5.59 762 71 33 1011
35 predicted protein [Populus trichocarpa] Glyma36750.1 XP_002327728 99030 5.56 999 130 26 1624
36 hypothetical protein [Vitis vinifera] Glyma16g32960.1 XP_002267091 48231 6.06 1216 56 40 842
37 decarboxylase isozyme 1 [Glycine max] Glyma13g30490.1 P51850 64169 5.73 626 97 35 1060
38 Ran3A-1 [Dimocarpus longan] Glyma04g07350.1 AEM97804 25505 6.38 914 86 53 427
39 copper amino oxidase [Glycine max] Glyma17g02260.1 CAE47488 76061 6.21 696 121 32 1284
40 stem 28 kDa glycoprotein [Glycine max] Glyma07g01730.1 P15490 29218 8.75 1419 108 51 528
41 (No significant hits to report)
42 alcohol dehydrogenase-1F [Phaseolus acutifolius] Glyma04g41990.1 Caa80691 41638 5.97 2015 183 53 734
43 methionine synthase [Glycine max] Glyma16g04240.1 AAQ08403 84401 5.93 2071 243 48 1419
44 hypothetical protein [Vitis vinifera] Glyma08g13440.1 XP_002270155 39049 5.12 2842 178 42 417
45 hypothetical protein [Vitis vinifera] Glyma13g41960.1 XP_002268097 35546 5.29 3702 207 58 560
46 cytosolic glutamine synthetase GSbeta1 [Glycine max] Glyma11g33560.1 AAG24873 39138 5.48 262 20 27 718
47 heat shock 70 kDa protein [Glycine max] Glyma08g06950.1 Q01899 74981 6.02 3835 187 39 1157
48 lactoylglutathione lyase [Gossypium hirsutum] Glyma09g00660.1 ACJ11750 32504 5.76 645 70 31 531
49 glutamate-1-Semialdehyde2,1-aminomutase [Glycine max] Glyma04g00420.1 P45621 50239 6.05 168 7 12 874
50 predicted protein [Populus trichocarpa] Glyma02g13330.1 XP_002305394 45064 6.10 2165 185 40 685
51 dihydrolipoamide acetyltransferase [Cucumis melo] Glyma07g03930.1 ADN33731 59813 8.00 109 10 16 697
52 predicted protein [Populus trichocarpa] Glyma09g40690.1 XP_002323696 61059 5.51 2019 157 34 949
53 6-phosphogluconate dehydrogenase [Glycine max] Glyma18g51260.1 BAA22812 53903 5.68 699 93 27 927
54 glutathione reductase [Vigna unguiculata] Glyma16g27210.1 ABB89042 54243 5.63 1795 168 35 880
55 d-3-phosphoglycerate dehydrogenase [Ricinus communis] Glyma10g40750.1 XP_002518687 62859 6.32 41 2 14 878
56 (No significant hits to report)
57 argininosuccinate synthase,putative [Ricinus communis] Glyma05g03190.1 XP_002521168 52641 6.55 16 2 13 774
58 pyruvate decarboxylase 1 [Lotus corniculatus] Glyma18g43460.1 AAO72533 66371 5.80 273 46 19 1071
59 Chalcone-flavonone isomerase 1A [Glycine max] Glyma20g38560.1 Q93XE6 23307 6.23 2359 126 61 394
60 6-phosphogluconate dehydrogenase [Glycine max] Glyma08g28230.1 BAA22812 53811 6.11 1433 110 39 899
61 Os05g0553000 [Oryza sativa] Glyma10g41330.1 NP_001056261 59913 5.80 39 2 15 879
62 predicted protein [Populus trichocarpa] Glyma11g03330.1 XP_002329902 65864 6.26 585 52 39 854
63 (No significant hits to report)
64 quinine oxidoreductase [Cicer arietinum] Glyma13g32300.1 CAD31838 21653 6.43 1017 80 47 384
65 ald/keto reductase,putative [Ricinus communis] Glyma03g40680.1 XP_002512220 37793 6.26 201 22 33 510
66 Knitz trypsin protease inhibitor [Glycine max] Glyma09g28310.1 ACA23207 22947 5.24 405 37 33 397
67 peroxisomal betaine-aldehyde dehydrogenase [Glycine max] Glyma06g19820.1 BAG09377 55389 5.23 965 111 35 1015
68 ripening related protein [Glycine max] Glyma08g24760.1 AAD50376 17865 5.96 1338 195 79 263
69 peptidyl-prolyl cis-trans isomerase 1 [Glycine max] Glyma11g10480.1 Q8W171 18441 8.70 1440 71 62 321
70 Os05g0553000 [Oryza sativa] Glyma10g41330.1 NP_001056261 59913 5.80 3205 160 50 879
71 S-adenosylmethionine synthetase [Cicer arietinum] Glyma15g21890.1 ACL14491 43425 5.50 1599 98 51 745
72 (No significant hits to report)
73 fructose-bisphosphate aldolase [Glycine max] Glyma02g38730 O65735 38469 7.12 2591 148 76 634

Spot no shows Spot number. Protein ID shows Phytozome protein ID. Accession no shows NCBI Accession number. MP shows matched peptides. Cov shows sequence coverage.