Table 1. Flooding-responsive proteins identified in soybean root using LC-MS/MS.
| Theoretical | ||||||||||
| Spot no | Homologous protein | Species | Protein ID | Accession no | Mr (Da) | pI | Score | MP | Cov(%) | BlastScore |
| 1 | Kunitz trypsin protease inhibitor | [Glycine max] | Glyma09g28310.1 | ACA23207 | 22947 | 5.24 | 28 | 2 | 15 | 397 |
| 2 | predicted protein | [Populus trichocarpa] | Glyma03g34820.1 | XP_002327377 | 20260 | 6.13 | 26 | 2 | 15 | 212 |
| 3 | (No significant hits to report) | |||||||||
| 4 | enolase | [Glycine max] | Glyma03g34830.1 | AAS18240 | 47914 | 5.49 | 72 | 2 | 13 | 863 |
| 5 | Heat shock 70 kDa protein | [Glycine max] | Glyma17g08020.1 | P26413 | 71220 | 5.28 | 2152 | 25 | 38 | 1144 |
| 6 | U2 small nuclear ribonucleoprotein A | [Ricinus communis] | Glyma20g18440.1 | XP_002512712 | 32537 | 5.16 | 104 | 3 | 18 | 405 |
| 7 | UDP-glucose 6-dehydrogenase | [Glycine max] | Glyma08g26520.1 | Q96558 | 53478 | 5.74 | 66 | 2 | 14 | 936 |
| 8 | dihydroxyacid dehydratase | [Glycine max] | Glyma13g27810.1 | ACU26534 | 64644 | 5.76 | 314 | 23 | 25 | 1160 |
| 9 | V- ATPase subnit A | [Glycine max] | Glyma08g23990.1 | ABU87506 | 69020 | 5.48 | 281 | 47 | 26 | 1217 |
| 10 | predicted protein | [Populus trichocarpa] | Glyma09g40690.1 | XP_002323696 | 61059 | 5.51 | 1049 | 104 | 30 | 949 |
| 11 | oligopeptidase A | [Medicago truncatula] | Glyma01g02480.1 | ABY48141 | 88745 | 6.03 | 777 | 84 | 43 | 1227 |
| 12 | Proteasome subnit alpha type-6 | [Glycine max] | Glyma06g39710.1 | O48551 | 27481 | 5.58 | 1406 | 107 | 47 | 489 |
| 13 | glutathione reductase | [Vigna unguiculata] | Glyma16g27210.1 | ABB89042 | 54243 | 5.63 | 629 | 79 | 48 | 880 |
| 14 | hypothetical protein | [Vitis vinifera] | Glyma06g48360.1 | XP_002272730 | 67578 | 5.60 | 765 | 89 | 39 | 1075 |
| 15 | isoflavonoid malonyl transferase 2 | [Medicago truncatula] | Glyma18g49240.1 | ABY91222 | 56964 | 5.70 | 601 | 75 | 29 | 430 |
| 16 | predicted protein | [Populus trichocarpa] | Glyma05g37670.1 | XP_002298026 | 112704 | 5.89 | 69 | 9 | 15 | 1470 |
| 17 | hypothetical protein | [Vitis vinifera] | Glyma18g04730.1 | XP_002270157 | 82072 | 6.21 | 225 | 15 | 17 | 1216 |
| 18 | UDP-D-apiose/xylose synthetase | [Gossypium hirsutum] | Glyma11g19550.1 | ACJ11753 | 43680 | 5.83 | 283 | 47 | 27 | 728 |
| 19 | hypothetical protein | [Vitis vinifera] | Glyma02g36530.1 | XP_002276114 | 60039 | 6.69 | 70 | 7 | 16 | 854 |
| 20 | isocitrate dehydrogenase [NADP] | [Glycine max] | Glyma02g40820.1 | Q06197 | 46393 | 5.87 | 1474 | 124 | 40 | 828 |
| 21 | UDP-glucose 6-dehydrogenase | [Glycine max] | Glyma08g26520.1 | Q96558 | 53478 | 5.74 | 141 | 8 | 19 | 936 |
| 22 | seed maturation protein PM31 | [Glycine max] | Glyma02g08400.1 | AAD30865 | 17907 | 6.10 | 57 | 2 | 19 | 323 |
| 23 | elongation factor 1-gamma | [Glycine max] | Glyma16g00360.1 | AAL82617 | 47785 | 5.92 | 117 | 2 | 15 | 619 |
| 24 | nucleoside diphosphate kinase | [Glycine max] | Glyma03g25820.1 | AAN77501 | 16308 | 6.30 | 76 | 2 | 12 | 284 |
| 25 | cysteine proteinase inhibitor | [Glycine max] | Glyma13g25870.1 | BAA19608 | 27708 | 6.57 | 57 | 5 | 10 | 417 |
| 26 | Transaminase mtnE | [Ricinus communis] | Glyma08g06790.1 | XP_002522052 | 50463 | 6.67 | 607 | 54 | 32 | 721 |
| 27 | acetoacetyl-CoA thiolase | [Medicago sativa] | Glyma14g00760.1 | ACX47470 | 41930 | 6.16 | 469 | 46 | 39 | 678 |
| 28 | hypothetical protein isoform 2 | [Vitis vinifera] | Glyma01g06970.1 | XP_002269733 | 53522 | 6.34 | 448 | 44 | 38 | 890 |
| 29 | acetoacetyl-CoA thiolase | [Medicago sativa] | Glyma14g00760.1 | ACX47470 | 41930 | 6.16 | 62 | 2 | 13 | 678 |
| 30 | Gamma-glutamyl hydrolase | [Glycine max] | Glyma13g34290.1 | P93164 | 38282 | 6.72 | 3208 | 212 | 36 | 619 |
| 31 | serine hydroxymethyltransferase 2 | [Glycine max] | Glyma05g28490. | ACM45952 | 52142 | 6.90 | 982 | 67 | 25 | 920 |
| 32 | heat shock protein 70 | [Gossypium hirsutum] | Glyma19g35560.1 | ACJ11741 | 71854 | 5.05 | 3704 | 251 | 37 | 1135 |
| 33 | actin isoform PEAc14-1 | [Pisum sativum] | Glyma02g10170.1 | ADP09679 | 42036 | 5.23 | 682 | 107 | 51 | 754 |
| 34 | glutamate decarboxylase | [Glycine max] | Glyma18g04940.1 | BAF80896 | 57554 | 5.59 | 762 | 71 | 33 | 1011 |
| 35 | predicted protein | [Populus trichocarpa] | Glyma36750.1 | XP_002327728 | 99030 | 5.56 | 999 | 130 | 26 | 1624 |
| 36 | hypothetical protein | [Vitis vinifera] | Glyma16g32960.1 | XP_002267091 | 48231 | 6.06 | 1216 | 56 | 40 | 842 |
| 37 | decarboxylase isozyme 1 | [Glycine max] | Glyma13g30490.1 | P51850 | 64169 | 5.73 | 626 | 97 | 35 | 1060 |
| 38 | Ran3A-1 | [Dimocarpus longan] | Glyma04g07350.1 | AEM97804 | 25505 | 6.38 | 914 | 86 | 53 | 427 |
| 39 | copper amino oxidase | [Glycine max] | Glyma17g02260.1 | CAE47488 | 76061 | 6.21 | 696 | 121 | 32 | 1284 |
| 40 | stem 28 kDa glycoprotein | [Glycine max] | Glyma07g01730.1 | P15490 | 29218 | 8.75 | 1419 | 108 | 51 | 528 |
| 41 | (No significant hits to report) | |||||||||
| 42 | alcohol dehydrogenase-1F | [Phaseolus acutifolius] | Glyma04g41990.1 | Caa80691 | 41638 | 5.97 | 2015 | 183 | 53 | 734 |
| 43 | methionine synthase | [Glycine max] | Glyma16g04240.1 | AAQ08403 | 84401 | 5.93 | 2071 | 243 | 48 | 1419 |
| 44 | hypothetical protein | [Vitis vinifera] | Glyma08g13440.1 | XP_002270155 | 39049 | 5.12 | 2842 | 178 | 42 | 417 |
| 45 | hypothetical protein | [Vitis vinifera] | Glyma13g41960.1 | XP_002268097 | 35546 | 5.29 | 3702 | 207 | 58 | 560 |
| 46 | cytosolic glutamine synthetase GSbeta1 | [Glycine max] | Glyma11g33560.1 | AAG24873 | 39138 | 5.48 | 262 | 20 | 27 | 718 |
| 47 | heat shock 70 kDa protein | [Glycine max] | Glyma08g06950.1 | Q01899 | 74981 | 6.02 | 3835 | 187 | 39 | 1157 |
| 48 | lactoylglutathione lyase | [Gossypium hirsutum] | Glyma09g00660.1 | ACJ11750 | 32504 | 5.76 | 645 | 70 | 31 | 531 |
| 49 | glutamate-1-Semialdehyde2,1-aminomutase | [Glycine max] | Glyma04g00420.1 | P45621 | 50239 | 6.05 | 168 | 7 | 12 | 874 |
| 50 | predicted protein | [Populus trichocarpa] | Glyma02g13330.1 | XP_002305394 | 45064 | 6.10 | 2165 | 185 | 40 | 685 |
| 51 | dihydrolipoamide acetyltransferase | [Cucumis melo] | Glyma07g03930.1 | ADN33731 | 59813 | 8.00 | 109 | 10 | 16 | 697 |
| 52 | predicted protein | [Populus trichocarpa] | Glyma09g40690.1 | XP_002323696 | 61059 | 5.51 | 2019 | 157 | 34 | 949 |
| 53 | 6-phosphogluconate dehydrogenase | [Glycine max] | Glyma18g51260.1 | BAA22812 | 53903 | 5.68 | 699 | 93 | 27 | 927 |
| 54 | glutathione reductase | [Vigna unguiculata] | Glyma16g27210.1 | ABB89042 | 54243 | 5.63 | 1795 | 168 | 35 | 880 |
| 55 | d-3-phosphoglycerate dehydrogenase | [Ricinus communis] | Glyma10g40750.1 | XP_002518687 | 62859 | 6.32 | 41 | 2 | 14 | 878 |
| 56 | (No significant hits to report) | |||||||||
| 57 | argininosuccinate synthase,putative | [Ricinus communis] | Glyma05g03190.1 | XP_002521168 | 52641 | 6.55 | 16 | 2 | 13 | 774 |
| 58 | pyruvate decarboxylase 1 | [Lotus corniculatus] | Glyma18g43460.1 | AAO72533 | 66371 | 5.80 | 273 | 46 | 19 | 1071 |
| 59 | Chalcone-flavonone isomerase 1A | [Glycine max] | Glyma20g38560.1 | Q93XE6 | 23307 | 6.23 | 2359 | 126 | 61 | 394 |
| 60 | 6-phosphogluconate dehydrogenase | [Glycine max] | Glyma08g28230.1 | BAA22812 | 53811 | 6.11 | 1433 | 110 | 39 | 899 |
| 61 | Os05g0553000 | [Oryza sativa] | Glyma10g41330.1 | NP_001056261 | 59913 | 5.80 | 39 | 2 | 15 | 879 |
| 62 | predicted protein | [Populus trichocarpa] | Glyma11g03330.1 | XP_002329902 | 65864 | 6.26 | 585 | 52 | 39 | 854 |
| 63 | (No significant hits to report) | |||||||||
| 64 | quinine oxidoreductase | [Cicer arietinum] | Glyma13g32300.1 | CAD31838 | 21653 | 6.43 | 1017 | 80 | 47 | 384 |
| 65 | ald/keto reductase,putative | [Ricinus communis] | Glyma03g40680.1 | XP_002512220 | 37793 | 6.26 | 201 | 22 | 33 | 510 |
| 66 | Knitz trypsin protease inhibitor | [Glycine max] | Glyma09g28310.1 | ACA23207 | 22947 | 5.24 | 405 | 37 | 33 | 397 |
| 67 | peroxisomal betaine-aldehyde dehydrogenase | [Glycine max] | Glyma06g19820.1 | BAG09377 | 55389 | 5.23 | 965 | 111 | 35 | 1015 |
| 68 | ripening related protein | [Glycine max] | Glyma08g24760.1 | AAD50376 | 17865 | 5.96 | 1338 | 195 | 79 | 263 |
| 69 | peptidyl-prolyl cis-trans isomerase 1 | [Glycine max] | Glyma11g10480.1 | Q8W171 | 18441 | 8.70 | 1440 | 71 | 62 | 321 |
| 70 | Os05g0553000 | [Oryza sativa] | Glyma10g41330.1 | NP_001056261 | 59913 | 5.80 | 3205 | 160 | 50 | 879 |
| 71 | S-adenosylmethionine synthetase | [Cicer arietinum] | Glyma15g21890.1 | ACL14491 | 43425 | 5.50 | 1599 | 98 | 51 | 745 |
| 72 | (No significant hits to report) | |||||||||
| 73 | fructose-bisphosphate aldolase | [Glycine max] | Glyma02g38730 | O65735 | 38469 | 7.12 | 2591 | 148 | 76 | 634 |
Spot no shows Spot number. Protein ID shows Phytozome protein ID. Accession no shows NCBI Accession number. MP shows matched peptides. Cov shows sequence coverage.