Table 2. Flooding-responsive proteins identified in soybean cotyledon using LC-MS/MS.
| Theoretical | ||||||||||
| Spot no | Homologous protein | Species | Protein ID | Accession no | Mr (Da) | pI | Score | MP | Cov (%) | Blast Score |
| 1 | sucrose-binding protein | [Glycine max] | Glyma10g03390.1 | Q04672 | 58353 | 6.08 | 22 | 3 | 17 | 723 |
| 2 | (No significant hits to report) | |||||||||
| 3 | lactoylglutathione lyase | [Gossypium hirsutum] | Glyma09g00660.1 | ACJ11750 | 32504 | 5.76 | 393 | 15 | 21 | 531 |
| 4 | (No significant hits to report) | |||||||||
| 5 | glycinin G2 | [Glycine max] | Glyma03g32020.1 | P04405 | 54927 | 5.46 | 202 | 26 | 20 | 799 |
| 6 | NADPH-protochlorophyllide oxidoreductase | [Vigna radiata] | Glyma06g38160.1 | AAF89208 | 43247 | 9.12 | 132 | 15 | 19 | 714 |
| 7 | (No significant hits to report) | (No significant hits to report) | ||||||||
| 8 | beta-conglycinin alpha prime subunit | [Glycine max] | Glyma10g39150.1 | BAE02726 | 72469 | 5.50 | 105 | 19 | 15 | 893 |
| 9 | beta-conglycinin alpha subunit | [Glycine max] | Glyma20g28650.1 | BAE44299 | 70549 | 5.12 | 82 | 22 | 17 | 783 |
| 10 | (No significant hits to report) | |||||||||
| 11 | beta-conglycinin alpha subunit | [Glycine max] | Glyma20g28650.1 | BAE44299 | 70549 | 5.12 | 121 | 33 | 17 | 783 |
| 12 | hypothetical protein | [Vitis vinifera] | Glyma04g35950.1 | XP_002281671 | 91567 | 5.11 | 63 | 6 | 11 | 1446 |
| 13 | predicted protein | [Populus trichocarpa] | Glyma01g05060.1 | XP_002323767 | 48123 | 5.61 | 81 | 2 | 13 | 539 |
| 14 | cytosolic malate dehydrogenase | [Glycine max] | Glyma02g00810.1 | AAS18241 | 35787 | 5.91 | 140 | 7 | 13 | 631 |
| 15 | heat shock 70 kDa protein | [Glycine max] | Glyma17g08020.1 | P26413 | 71220 | 5.28 | 130 | 16 | 24 | 1144 |
| 16 | sucrose-binding protein | [Glycine max] | Glyma10g03390.1 | Q04672 | 58353 | 6.08 | 475 | 38 | 14 | 723 |
| 17 | (No significant hits to report) | |||||||||
| 18 | (No significant hits to report) | |||||||||
| 19 | glyoxysomal isocitrate lyase isoform 1 | [Glycine max] | Glyma06g45950.1 | ABD28288 | 65144 | 7.26 | 30 | 2 | 12 | 1075 |
| 20 | (No significant hits to report) | |||||||||
| 21 | ferrous iron transport protein b | [Ralstonia solanacearum] | Glyma02g08980.1 | AEG70893 | 217645 | 5.32 | 21 | 2 | 10 | 22.7 |
| 22 | glycinin G2[Glycine max] | [Glycine max] | Glyma03g32030.1 | P04405 | 54927 | 5.46 | 382 | 23 | 19 | 799 |
| 23 | sucrose-binding protein | [Glycine max] | Glyma10g03390.1 | Q04672 | 58353 | 6.08 | 1359 | 102 | 36 | 723 |
| 24 | glycinin G3 | [Glycine max] | Glyma19g34780.1 | P11828 | 54835 | 5.73 | 53 | 9 | 16 | 796 |
| 25 | (No significant hits to report) | |||||||||
| 26 | sucrose-binding protein | [Glycine max] | Glyma10g03390.1 | Q04672 | 58353 | 6.08 | 2101 | 142 | 54 | 723 |
| 27 | beta-conglycinin alpha subunit | [Glycine max] | Glyma20g28650.1 | BAE44299 | 70549 | 5.12 | 584 | 96 | 33 | 783 |
| 28 | ferrous iron transport protein b | [Ralstonia solanacearum] | Glyma03g42290.1 | AEG70893 | 217201 | 6.16 | 15 | 2 | 10 | 22.7 |
Spot no shows Spot number. Protein ID shows Phytozome protein ID. Accession no shows NCBI Accession number. MP shows matched peptides. Cov shows sequence coverage.