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. 2013 Jun 14;8(6):e65605. doi: 10.1371/journal.pone.0065605

Figure 4. Amino acid preferences of the AIDA1 PTB domain for APP determined from a peptide array.

Figure 4

A list of peptides on the array are provided in supplementary material. (a) The array probed with anti 6xHis mAb only. Positive control 6xHis peptides are identified by a +. (b) The array probed with 6xHis-AIDA1 PTB domain. (c) Sliding window peptide scan of 12-mers spanning aa. 672–697 of APP. Peptides are duplicated on the array; for example, at A3 and A18. Since peptide content per spot can vary, if a signal was observed at the exposure presented it was deemed to be interaction. (d) Results of a window scan across the APP C-terminal sequence and an exhaustive positional scan. Grey boxes indicate binding was observed, regardless of signal intensity.