Table 4. Affnities of APP-derived peptides for two solubility enhanced mutants of the AIDA1 PTB domain. ND: not done.
Ligand | Peptide sequence | Kd (µM) | |
PTBY70A | PTB5M | ||
APP-32 | ggDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN | 13.3±1.5 (n = 4) | 14.8±1.0 (n = 4) |
APP-17 | ggQNGYENPTYKFFEQMQN | 11.3±1.9 (n = 2) | 11.5±1.4 (n = 2) |
APP-17{pY} | ggQNGYENPT{pY}KFFEQMQN | ND | >1000(n = 1) |