TABLE 1.
Effect of inoculum size on cell morphology in C. ulmi at 20 h of growth
Inoculum size cells/ml | % of mycelia with growth ona:
|
|
---|---|---|
GPP | GPR | |
2 × 107 | 16 | 95 |
1 × 107 | 15 | 95 |
8 × 106 | 20 | 95 |
6 × 106 | 20 | 95 |
4 × 106 | 20 | 95 |
2 × 106 | 18 | 95 |
1 × 106 | 27 | 95 |
4 × 105 | 95 | 95 |
1 × 105 | 95 | 95 |
4 × 104 | 95 | 95 |
Filamentous growth is given. Only differentiated cells were counted; thus, the percentages of mycelial-filamentous growth and percentage of yeasts sum as 100%. GPP medium contains proline as the nitrogen source, whereas GPR medium contains arginine. Values shown are averages of data from triplicate experiments performed earlier (14). Similar results agreeing with ±10% were obtained by four researchers using two strains (6404 and 6405) over a span of 20 years.