Table 2. Kinetic parameters^a of recombinant laccases on benchmark substrates (mean ± SD).

	Lcc4A			Lcc5			Lcc7			Lcc1A			Lcc1B
Substrate	Km (µM)	Vmax (U mg−1)b	Vmax/Km (U mg−1 M−1)b	Km (µM)	Vmax (U mg−1)b	Vmax/Km (U mg−1 M−1)b	Km (µM)	Vmax (U mg−1)b	Vmax/Km (U mg−1 M−1)b	Km (µM)	Vmax (U mg−1)b	Vmax/Km (U mg−1 M−1)b	Km (µM)	Vmax (U mg−1)b	Vmax/Km (U mg−1 M−1)b
ABTS	11.2±0.9	0.61±0.01	(5.44±0.31)×104	325±47	2.26±0.36	(6.94±0.16) ×103	36.3±1.9	19.7±0.6	(5.43±0.23) ×105	14.3±1.9	2.65±0.65	(1.92±0.75)×105	42.5±10.3	5.61±2.26	(1.28±0.22)×105
CAT	255±76	0.91±0.11	(3.72±0.75)×103	6280±680	2.65±0.10	(4.24±0.31) ×102	38800±5300	0.53±0.05	13.8±0.08	2030±140	0.62±0.13	(3.08±0.85)×102	3540±230	0.94±0.31	(2.69±0.95)×102
DMP	26.0±2.4	0.26±0.03	(1.00±0.03)×104	113±8	0.60±0.03	(5.30±0.28) ×103	12000±1000	1.73±0.09	(1.44±0.05) ×102	120±4	1.08±0.24	(8.94±1.79)×103	115±17	1.41±0.52	(1.20±0.29)×104
DOPA	1040±150	1.07±0.10	(1.03±0.05)×103	10500±700	1.66±0.15	(1.58±0.07) ×102	n.a.c	n.a.	n.a.	4550±570	0.49±0.19	(1.06±0.28)×102	5520±850	0.65±0.15	(1.22±0.45)×102
GUA	648±105	0.16±0.01	(2.46±0.27)×102	1270±90	0.32±0.01	(2.57±0.17) ×102	8630±2120	0.18±0.05	21.4±0.3	943±58	0.73±0.13	(7.85±1.72)×102	913±68	0.96±0.25	(1.07±0.35)×103
SGZ	n.d.d	0.09±0.01	n.d.	n.d.	0.02±0.00	n.d.	n.d.	2.52±0.81	n.d.	n.d.	0.98±0.20	n.d.	n.d.	0.75±0.07	n.d.
TYR	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.	n.a.

^aApparent values. Parameters of Lcc1A and Lcc1B were obtained from our previous study [2].

^bOne U was defined as the number of µmol of respective oxidized product formed in one minute under standard assay condition.

^cNo measurable activity.

^dNot determined.