Figure 3.

CCR5 expression by T cells and putative DCs in the human rectum and colon. A: Representative 20x pseudo-color images in one individual of CD3 (white) and CCR5 (red) co-staining in the rectum and colon. Tissue sections were stained as described in Methods. Mouse IgG isotype controls for CCR5 staining are shown in Figure 2A. Corresponding dot plots are shown next to each image and depict CCR5 mean and maximum fluorescence intensities of cells that were first gated to stain positive for CD3. Thus, cells within the upper right quadrants of the dot plots co-express CD3 and CCR5. The numbers of CD3⁺ CCR5⁺ cells in the upper right quadrants and their corresponding cell densities are specified in each plot. Also given is the percentage of CD3⁺ T cells co-expressing CCR5. B: Representative 20x pseudo-color images and dot plots in one individual of CD209 (white) and CCR5 (red) co-staining in the rectum and colon. C: Summary of percentages (left y axis) of CD3⁺ T cells co-expressing CCR5 and their cell densities (right y axis) in all biopsies analyzed. D: Summary of percentages (left y axis) of CD209⁺ cells co-expressing CCR5 and their cell densities (right y axis) in all biopsies analyzed. In C and D, paired samples, i.e., where the data were derived from a colon and rectum biopsy obtained from the same individual at the same time point, are connected by lines. Differences going against the prevailing trend are indicated by solid lines, others by dotted lines.