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. 2013 Jun 14;8(6):e68065. doi: 10.1371/journal.pone.0068065

Figure 8. FKHR-PAX3 selectively increased cell adhesion over cell movement and invasion.

Figure 8

(A) FKHR-PAX3 increased attachment of RD (top panel) and RH30 (bottom panel) cells to the indicated extracellular matrix components. (B) FKHR-PAX3 expression in RD (left panel) and RH30 (right panel) increased the strength of cell adhesion to culture dishes as indicated by a reduced sensitivity to trypsin. (C) Western blot analysis of the effect of ectopic FKHR-PAX3 expression on FAK phosphorylation in RD and RH30 cells. A total of 30 μg of whole cell extracts were analyzed. (D) FKHR-PAX3 reduced the migratory function in RD (top panel) and RH30 (bottom panel) cells as measured by scratch wound assay. Left panel: quantification of migratory index; right panel: representative micrographs of the scratched wound assays. (E) FKHR-PAX3 decreased the invasive potential of RD (left panel) and RH30 (right panel) as determined by Matrigel assay. ND: statistically no difference. (A-B, D-E) Assays were conducted as described in Materials and Methods. Representative data from FKHR-PAX3 isoform c is shown.