Figure 9. FKHR-PAX3 induced early onset of tumor formation in nude mice xenograft model.

(A) RD (top panel) and RH30 (bottom panel) cells expressing empty vector or FKHR-PAX3 were injected intramuscularly into the hind legs of nude mice as described in Materials and Methods. Data points represent mean ± s.d. of tumor volume (mm³) of all injected mice at the indicted time points. Inset: an expanded view over the early tumor development period. (B) Dissecting microscopic images (magnification 25X) of lung organs of two representative mice from the control (left) and FKHR-PAX3 (right) group, showing clear evidence of more extensive tumor mass and infiltration metastasis in the control group (red dashed lines: margins surrounding the tumor mass; blue arrow heads: light-reflective artifacts). Representative data from FKHR-PAX3 isoform c is shown.