TABLE 5.
Populations and reductions of stationary phase on E. coli O157:H7 cells treated with sanitizers as affected by temperature of pretreatment with 0.05% peptone or cleaners 5 and 7
| Treatment temp (°C) | Strain | Sanitizer | Population (log10 CFU/ml) and reduction after treatment with cleaners and sanitizersa
|
|||||
|---|---|---|---|---|---|---|---|---|
| Control
|
Cleaner 5
|
Cleaner 7
|
||||||
| Population | Reduction | Population | Reduction | Population | Reduction | |||
| 4 | EDL 933 | Control | a 8.38 | −0.23 a | a 8.33 | −0.23 a | a 4.23 | 0.25 a |
| Quorum Clear | c 2.51 | 5.64 a | c 3.33 | 4.78 b | b 0.63 | 3.84 b | ||
| Quorum Green | d <0.60 | 8.15 a | d 0.76 | 7.34 a | b <0.60 | 3.96 b | ||
| FS amine b | b 5.99 | 2.16 a | b 5.77 | 2.33 a | b 1.46 | 3.01 a | ||
| Cetylpyridinium chloride | b 6.15 | 1.99 a | b 5.41 | 2.69 a | b 1.48 | 2.99 a | ||
| Benzalkonium chloride | a 8.28 | −0.13 b | a 8.23 | −0.12 b | a 3.87 | 0.60 a | ||
| FRIK 816-3 | Control | a 8.36 | −0.62 c | a 8.31 | −0.01 a | a 1.79 | −0.39 b | |
| Quorum Clear | d 2.19 | 5.55 a | c 3.03 | 5.20 a | b <0.60 | 2.19 a | ||
| Quorum Green | c <0.60 | 7.74 a | d <0.60 | 8.29 a | b <0.60 | 2.19 b | ||
| FS amine b | bc 6.85 | 0.89 a | b 6.04 | 2.26 a | b <0.60 | 2.19 a | ||
| Cetylpyridinium chloride | c 6.45 | 1.29 a | b 6.07 | 2.22 a | b <0.60 | 1.66 a | ||
| Benzalkonium chloride | a 8.18 | −0.44 a | a 8.39 | −0.09 a | a 3.39 | −0.07 a | ||
| 12 | EDL 933 | Control | a 8.28 | −0.38 b | a 8.26 | −0.38 b | a 3.77 | 0.02 a |
| Quorum Clear | c 3.81 | 4.11 a | e 2.49 | 5.38 a | b <0.60 | 3.28 a | ||
| Quorum Green | d 1.38 | 6.54 a | f <0.60 | 7.97 a | b <0.60 | 3.23 b | ||
| FS amine b | b 5.70 | 2.21 b | c 5.79 | 2.04 b | b <0.60 | 3.56 a | ||
| Cetylpyridinium chloride | b 5.90 | 2.01 b | d 5.50 | 2.37 b | b <0.60 | 3.73 a | ||
| Benzalkonium chloride | a 7.71 | 0.20 b | b 7.81 | 0.06 b | a 2.99 | 0.80 a | ||
| FRIK 816-3 | Control | a 8.53 | −0.20 a | a 8.38 | −0.07 a | a 3.11 | 0.17 a | |
| Quorum Clear | c 3.25 | 5.08 a | c 3.46 | 4.85 a | b <0.60 | 3.28 a | ||
| Quorum Green | d <0.60 | 8.31 a | d <0.60 | 8.17 a | b <0.60 | 3.17 b | ||
| FS amine b | b 5.58 | 2.74 a | d <0.60 | 3.28 a | b <0.60 | 2.41 a | ||
| Cetylpyridinium chloride | b 5.30 | 3.02 a | b 6.03 | 2.29 a | b <0.60 | 3.28 a | ||
| Benzalkonium chloride | a 8.33 | −0.01 a | a 8.41 | −0.01 a | a 3.28 | 0.00 a | ||
a
The detection limit was 4 CFU/ml (0.60 log10 CFU/ml). Within treatment temperature, strain, and control or cleaner treatment, mean values that are not preceded by the same letter indicate significant differences caused by sanitizer treatment (P ≤ 0.05). Mean values of reductions in the same row that are not followed by the same letter indicate significant differences (P ≤ 0.05). Reductions indicate the differences between populations after pretreatment with 0.05% peptone (control) or cleaner and population after treatment with sterile water (control) or sanitizer.