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. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Neuron. 2013 Jun 5;78(5):799–806. doi: 10.1016/j.neuron.2013.04.003

Figure 4. Endogenous GABAdesychronizes circadian cells in the SCN.

Figure 4

(A) Representative traces of raw bioluminescence from two Vip−/−;PER2::LUC SCN explants show that gabazine (Gbz, 100 µM) rescued circadian rhythmicity compared to vehicle. (B) In the absence of VIP-signaling, gabazine increased the amplitude of the dominant period compared to baseline while vehicle treatment did not (n=10 explants in each condition; * indicates P<0.05). Total explant bioluminescence (C) and synchrony among cells (D,E) imaged from a Vip−/−;PER2::LUC explant (inset in C) quickly damped over 6 days of baseline recording while gabazine prevented damping of the bioluminescence rhythm and maintained circadian synchrony of the population.