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. 2004 Mar;70(3):1287–1296. doi: 10.1128/AEM.70.3.1287-1296.2004

FIG. 2.

FIG. 2.

Phylogenetic relationships of rhizobial FnrN and FixK proteins. The following proteins were included in the analysis: R. etli CNPAF512 FnrN (CNPFnrN); R. etli CFN42 FnrNd (CFNFnrNd) (accession number gi12083693) (31); R. leguminosarum bv. viciae strain VF39 FnrN (VF3FnrN) (accession number gi120623) (6); R. leguminosarum bv. viciae UPM791 FnrN1 (UPMFnrN1) (accession number gi619722) (23); R. leguminosarum bv. viciae UPM791 FnrN2 (UPMFnrN2) (accession number gi2114416 (22); R. etli CFN42 FnrNchr (CFNFnrNch) (accession number gi3462873) (31); B. japonicum FixK1 (BjaFixK1) (accession number gi95165) (1); M. loti strain MAFF303099 mll6632 (Mlomll6632) (accession number gi13475537) (28); A. caulinodans FixK (AcaFixK) (gi38702) (27); B. japonicum FixK2 (BjaFixK2) (gi3021322) (37); S. meliloti FixK1 (SmeFixK1) (gi14523782) (3); S. meliloti FixK2 (SmeFixK2) (gi14523500) (2); R. etli CFN42 FixKf (CFNFixKf) (gi9857982) (21); R. etli CFN42 FixKd (CFNFixKd) (gi1679718) (47); R. leguminosarum bv. viciae VF39 FixK (VF3FixK) (gi1240048) (39); Rhizobium strain IC3342 orf4 (IC3342hy) (gi152262) (54); mlr6409 (Mlomlr6409) (gi13475362), mml6632 (Mlomll6632) (gi13475537), and mll6578 (Mlomll6578) (gi14026245) from M. loti strain MAFF303099 (28); M. loti strain R7A ML0019 (R7AML0019) (gi20803846) (51); SMa0662 (SmeSMa0662) (gi16262801) and Sma1141 (SmeSMa1141) (gi16263070) from S. meliloti (2); and bll7696 (Bjabll7696) (gi27382807), bll3466 (Bjabll3466) (gi27378577), and bll2109 (Bjabll2109) (gi27350363) from B. japonicum (29). Details of the construction are described in Materials and Methods. E. coli Fnr (EcoFnr) (gi26108071) and Pseudomonas stutzeri DnrD (StuDnrD) (gi4585795) were included to represent members of classes IA and IB, respectively, of the Crp-Fnr family. The numbers at the nodes indicate the percentages of trees in 1,000 bootstrap analyses that supported the topology.