TABLE 2.
Expression of S. meliloti fixN-lacZ in S. meliloti and R. etli
| Straina | Plasmid | β-Glucuronidase activity (Miller units)b
|
|
|---|---|---|---|
| Aerobic conditions | Microaerobic conditions | ||
| GMI347-CS112 | 30 (3) | 11 (9) | |
| GMI347-CS112 | pFAJ1172 | 25 (3) | 969 (151) |
| GMI347-CS112 | pFAJ1175 | 21 (9) | 407 (76) |
| CNPAF512 | pGMI931 | 27 (4) | 1,033 (36) |
| FAJ1182 | pGMI931 | 29 (6) | 23 (3) |
| FAJ1183 | pGMI931 | 22 (2) | 8 (2) |
a
The S. meliloti strain used was GMI347-CS112 (fixJ mutant, chromosomal fixN-lacZ), and the R. etli strains used were CNPAF512 (wild type), FAJ1182, and FAJ1183 (fnrN mutant).
b
The values in parentheses are standard deviations. The data are the means of at least four replicates, and the results were confirmed independently in separate experiments.