TABLE 4.
Expression of R. etli fnrN-gusA (pFAJ1178) in R. etli wild-type and mutant backgroundsa
| Strain | Relevant genotype | β-Glucuronidase activity (Miller units)a
|
||
|---|---|---|---|---|
| Aerobic conditions | Microaerobic conditions | Symbiotic conditions | ||
| CNPAF512 | Wild type | 79 (33) | 474 (175) | 1,025 (169) |
| Rp1000 | nifA | 51 (29) | 380 (71) | 1,033 (99) |
| FAJ1182 | fnrN | 40 (6) | 59 (17) | 82 (12) |
| FAJ1183 | fnrN | 23 (5) | 66 (12) | 50 (22) |
| FAJ1154 | rpoN1 | 81 (20) | 267 (67) | 1,039 (145) |
| FAJ1169 | rpoN2 | 56 (38) | 451 (104) | 968 (131) |
| RpFAJ1002 | fixL | 51 (29) | 470 (108) | 1,008 (194) |
| RpFAJ1004 | fixJ | 60 (24) | 453 (131) | NDb |
a
The symbiotic expression data are the means of at least 10 replicates, and the results were confirmed independently in separate experiments. The free-living expression data are the means of at least four replicates. The values in parentheses are standard deviations.
b
ND, not determined.