Table 2.

Steady-State Kinetic Parameters for dNTP Incorporation Opposite DNA Lesions by Vent (exo⁻)

template base	dNTP	Km (µM)	kcat (s−1)	kcat/Km (mM−1 s−1)	f ins a	relative effciencyb
G	A	2300 ± 400	0.0098 ± 0.0008	0.0043	0.00039	3.9 × 10−4
	T	2700 ± 400	0.043 ± 0.004	0.016	0.0015	1.5 × 10−3
	G	1200 ± 200	0.012 ± 0.0006	0.01	0.00091	9.1 × 10−4
	C	6.2 ± 1.7	0.065 ± 0.003	11	1	1
N2-MeG	A	3200 ± 400	0.0068 ± 0.0004	0.0021	0.1	1.9 × 10−4
	T	1800 ± 300	0.0021 ± 0.0001	0.0012	0.057	1.1 × 10−4
	G	84 ± 32	0.0052 ± 0.0005	0.062	3	5.6 × 10−3
	C	1300 ± 200	0.027 ± 0.001	0.021	1	1.9 × 10−3
N2-BzG	A	2500 ± 400	0.0053 ± 0.0005	0.0022	52	2.0 × 10−4
	T	2300 ± 500	0.0004 ± 0.000004	0.00017	4	1.5 × 10−5
	G	1600 ± 300	0.0006 ± 0.00005	0.0004	10	3.6 × 10−5
	C	2400 ± 600	0.0001 ± 0.000002	0.000042	1	3.8 × 10−6
O6-MeG	A	2800 ± 900	0.0029 ± 0.0005	0.0011	0.058	1.0 × 10−4
	T	380 ± 60	0.014 ± 0.0007	0.037	1.9	3.3 × 10−3
	G	2300 ± 200	0.0067 ± 0.0004	0.0029	0.15	2.6 × 10−4
	C	1200 ± 200	0.022 ± 0.002	0.019	1	1.7 × 10−3
O6-BzG	A	1500 ± 300	0.0017 ± 0.0001	0.0011	0.18	1.0 × 10−4
	T	960 ± 30	0.003 ± 0.0003	0.0031	0.51	2.8 × 10−4
	G	310 ± 150	0.0002 ± 0.00003	0.00064	0.1	5.8 × 10−5
	C	130 ± 60	0.0008 ± 0.00008	0.0061	1	5.5 × 10−4
AP site	A	430 ± 70	0.043 ± 0.002	0.099	40	9.0 × 10−3
	T	2100 ± 900	0.017 ± 0.003	0.0081	3.2	7.4 × 10−4
	G	2300 ± 700	0.043 ± 0.007	0.019	7.6	1.7 × 10−3
	C	830 ± 250	0.0021 ± 0.0002	0.0025	1	2.3 × 10−4

^aMisinsertion frequency, calculated by dividing k_cat/K_m for dNTP incorporation by the k_cat/K_m for dCTP incorporation opposite each template base.

^bRelative insertion efficiency, calculated by dividing k_cat/K_m for dNTP incorporation opposite each template base by the k_cat/K_m for dCTP incorporation opposite template G.