Table 3.

Steady-State Kinetic Parameters for dNTP Incorporation Opposite DNA Lesions by Dpo4

template base	dNTP	Km (µM)	kcat (s−1)	kcat/Km (mM−1 s−1)	fins a	relative effciencyb
G	A	880 ± 80	0.0028 ± 0.00009	0.0032	0.0001	1 × 10−4
	T	940 ± 220	0.021 ± 0.002	0.022	0.00071	7.1 × 10−4
	G	430 ± 50	0.0045 ± 0.0002	0.01	0.00032	3.2 × 10−4
	C	12 ± 3	0.38 ± 0.02	31	1	1
N2-MeG	A	790 ± 30	0.0027 ± 0.00004	0.0034	0.00015	1.1 × 10−4
	T	1300 ± 200	0.025 ± 0.002	0.019	0.00083	6.1 × 10−4
	G	250 ± 30	0.0049 ± 0.0002	0.019	0.00083	6.1 × 10−4
	C	4.1 ± 1.8	0.092 ± 0.006	23	1	7.4 × 10−1
N2-BzG	A	590 ± 90	0.0016 ± 0.00008	0.0026	0.0011	8.4 × 10−5
	T	110 ± 80	0.00019 ± 0.00005	0.0016	0.00067	5.2 × 10−5
	G	50 ± 16	0.0016 ± 0.0001	0.03	0.0125	9.7 × 10−4
	C	26 ± 7	0.0062 ± 0.004	2.4	1	7.7 × 10−2
O6-MeG	A	2200 ± 300	0.0036 ± 0.0002	0.0016	0.013	5.2 × 10−5
	T	3800 ± 1200	0.063 ± 0.01	0.017	0.14	5.5 × 10−4
	G	12 ± 5	0.00032 ± 0.00002	0.027	0.23	8.7 × 10−4
	C	45 ± 12	0.0055 ± 0.0004	0.12	1	3.9 × 10−3
O6-BzG	A	1200 ± 200	0.0012 ± 0.00007	0.001	0.0091	3.2 × 10−5
	T	870 ± 40	0.0023 ± 0.00004	0.0026	0.024	8.4 × 10−5
	G	77 ± 23	0.00055 ± 0.00004	0.0065	0.059	2.1 × 10−4
	C	52 ± 20	0.0057 ± 0.0005	0.11	1	3.5 × 10−3
AP site	A	1100 ± 200	0.021 ± 0.003	0.02	0.24	6.5 × 10−4
	T	8900 ± 800	0.025 ± 0.002	0.0028	0.034	9.0 × 10−5
	G	600 ± 140	0.0033 ± 0.0002	0.0055	0.066	1.8 × 10−4
	C	27 ± 8	0.0022 ± 0.0002	0.083	1	2.7 × 10−3

^aMisinsertion frequency, calculated by dividing k_cat/K_m for dNTP incorporation by the k_cat/K_m for dCTP incorporation opposite each template base.

^bRelative insertion efficiency, calculated by dividing k_cat/K_m for dNTP incorporation opposite each template base by the k_cat/K_m for dCTP incorporation opposite template G.