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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Neuron. 2013 Mar 14;78(1):65–80. doi: 10.1016/j.neuron.2013.02.029

Figure 8. Overexpression of VCP-CD or A232E inhibits clearance of damaged mitochondria.

Figure 8

A. Imaging VCP-EGFP and mCherry-Parkin in Mito-Cerulean stable MEFs 24h after addition of CCCP. Cells were transfected with VCP-wt, CD or disease mutant A232E -EGFP. Scale bars equal 10µm. B. Quantification of cells with mitochondrial clearance. Cells were prepared as described in A. At least 30 cells were counted for each sample. Errors bars indicate standard error from 3 independent replicates. C. Quantification of cells with residual aggregates of mitochondria. Cells were prepared as described in A. At least 30 cells were counted for each sample. Errors bars indicate standard error from 3 independent replicates. C2C12 cell data are shown in Supplemental Figure 5E–G.