Table 1.

UV-melting data for decoding region analogues.

Analogues	ΔG°37 (kcal/mol)	ΔΔG°37 (kcal/mol)	ΔH° (kcal/mol)	ΔS° (cal/K· mol)	Tm(°C)
E. coli analogues
C-C-U	−10.9 ± 0.2	0	−49 ± 5	−122 ± 15	65.6
m4Cm-C-U	−12.2 ± 0.2	−1.3	−61 ± 4	−156 ± 13	68.9
C-m5C-U	−10.3 ± 0.2	0.6	−48 ± 5	−120 ± 17	63.6
C-C-m3U	−10.7 ± 0.2	0.2	−51 ± 7	−129 ± 21	63.3
Cm-C-U	−11.5 ± 0.2	−0.6	−55 ± 5	−141 ± 17	66.3
m4Cm-m5C-U	−11.4 ± 0.2	−0.5	−58 ± 7	−149 ± 21	66.1
m4Cm-C-m3U	−11.3 ± 0.2	−0.4	−55 ± 7	−141 ± 21	66.3
C-m5C-m3U	−10.5 ± 0.2	0.4	−47 ± 6	−119 ± 20	65.4
m4Cm-m5C-m3U	−11.3 ± 0.2	−0.4	−57 ± 7	−148 ± 21	65.6
C. acetobutylicum	−11.4 ± 0.2	−0.5	−58 ± 7	−149 ± 21	66.1
T. thermophilus	−11.7 ± 0.2	−0.8	−61 ± 6	−159 ± 20	66.3

Buffer conditions: 20 mM sodium cacodylate, 1 M NaCl, 0.5 mM Na₂EDTA, pH 7.0.