Fig. 1.

Trans-differentiation of murine ATII cells to an ATI-like cell phenotype. (A) ATII cells were cultured on fibronectin-coated coverslips for the indicated times and immunofluorescence assay was used to detect expression of phenotypic marker proteins of ATII cells, LBP180, or ATI cells, T1α. Nuclei were stained with DAPI, inset panels. (B) ATII cells were lysed on the day of isolation (day 0) or cultured on fibronectin and lysed on the indicated days. Cell lysates were analyzed by Western blot analysis using antibody against T1α or β-actin, as a protein loading control. The images shown are representative of three replicate experiments.