Fig. 6.

Expression of IL-1β by primary murine alveolar epithelial cells in response to respiratory viral infection. Murine cells were cultured as an ATI (A) or ATII (B) cell phenotype, infected by the indicated viruses, and IL-1β mRNA was quantified by RT-qPCR. All ATII cell samples (B) were infected for 24 h. Mock-inoculated and LPS-treated cells were used as negative and positive controls, respectively. Expression of IL-1β was normalized to that of β-actin and the means and standard errors from 3 to 5 replicate experiments are shown. Statistically significant differences in expression compared to mock samples were determined by unpaired t test. *p < 0.05, **p < 0.005.