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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cytokine. 2013 May 8;63(1):6–9. doi: 10.1016/j.cyto.2013.04.015

Figure 2. IL-17 and IFN- production by superantigen stimulation and reduction by anti-MHC class-II and anti-TCR αβ chains.

Figure 2

(A) MOG35-55- and HSV-specific T cell clones were respectively stimulated by specific antigen (10 μg/ml MOG35-55 or HSV protein) or SEB and TSST-1 in the presence of T- and NK-deleted APC for 72 hours (pooled data of three experiments). (B) Anti-HLA DR, DP and DQ mixture at a total concentration of 20 μg/ml and 10 μg/ml anti-human TCR αβ chains were respectively added to Th1 clones under superantigen-stimulation for 72 hours. IFN- and IL-17 production in the supernatant was measured by ELISA (representative of two experiments). (C) ELISPOT assay showing IFN- and IL-17 producing cells stimulated by superantigen. SFC for IL-17+ in blue, IFN- + in red and IL-17+/IFN- + labeled in pink were numerated by CTL suing a quantitative SFC (representative of two experiments).