Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Int J Biochem Cell Biol. 2013 Apr 8;45(7):1274–1280. doi: 10.1016/j.biocel.2013.03.020

Figure 2.

Figure 2

Collagen blocks Notch regulation of smooth muscle promoters. Experiments were performed as in Fig 1 to determine the effects of type IV collagen on SMA and MHC promoters. A7R5 cells were cotransfected with either SMA-Luciferase (A) or MHC-Luciferase (B) plasmids and Renilla luciferase (as a transfection control). Cocultures were treated with increasing concentrations of type IV collagen. Smooth muscle promoter activation was determined using the ratio of firefly/Renilla luciferase activity. Collagen effectively inhibited expression of both promoter constructs. All groups included three replicates. * denotes significant differences between control and Notch ligand-stimulated cultures (p<0.05).