TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant propertiesa	Source or reference
Strains
Nocardia sp. strain NRRL 5646	Wild type	24
E. coli JM 109	RecA−, recombinant vector host strain	Promega
E. coli BL21 (DE3)	Inducible T7 RNA polymerase, Ampr	Stratagene
E. coli BL21-CodonPlus (DE3)-RP	Has argU and proL tRNA genes to provide tRNA for rare codons found in many GC-rich bacteria	Stratagene
Plasmids
pGEM-T easy	T/A PCR cloning vector, Ampr	Promega
pHAT10	Cloning vector for in-frame addition of HAT tag (MTMITPSLKDHLIHNVH KEEHAHAHNKIDDDDKVDGS) to the N terminus of CAR, pLac for expression, Ampr	Clontech
pHAT-305	pHAT-10 with car insert	This study
pHAT-DHFR	Positive control expression vector with dihydrofolate reductase gene tagged with HAT at the N terminus	Clontech

^aAmp^r, ampicillin resistance.