TABLE 2.
Oligonucleotides used in this study
| Oligo- nucleotide | Sequence (5′-3′)a | Source or reference |
|---|---|---|
| Cloning primers | ||
| CA-1 | GTSGATTCACCSGATGAG | This study |
| CA-2 | CCSGATGARCGSCTACAG | This study |
| CA-3 | TGSGCSACSGTSACGAAC | This study |
| CA-4 | SACGAAYTCSCCCTGSGAC | This study |
| CA-5 | GGTCGGGATCAATCTCAACTACATG | This study |
| CA-6 | CCTGCTCATCTTCTGCAAACAACTG | This study |
| carF | CGCGGATCCGCAGTGGATTCACCG GATGAG | This study |
| carR | CGGGGTACCCCTGATATCCGTCAG AGCAGCTG | This study |
| Sequencing primers | ||
| T7 | TAATACGACTCACTATAGGG | |
| SP6 | CATACGATTTAGGTGACACTATAG | Sigma-Genosys |
| M13 reverse | CAGGAAACAGCTATGAC | Sigma-Genosys |
| Scar-1 | CTCGACCTGGCCGATATCCAC | This study |
| Scar-2 | GAGGACGGCTTCTACAAGAC | This study |
| Scar-3 | GACGCGCACTTCACCGACCTG | This study |
| Scar-4 | GTCGACCTGATCGTCCATCC | This study |
| Sacr-5 | GACCTACGACGTGCTCAATC | This study |
| Scar-6 | CGTACGACGATGGCATCTC | This study |
| Scar-7 | GTGGATATCGGCCAGGTCGAG | This study |
| He-32 | GGTGGCAGGATGGAATCGG | This study |
| He-33 | CGTCGATTCGCGATTCCCTG | This study |
a
Restriction cleavage sites are underlined. R represents A or G, Y represents C or T, and S represents G or C.