Figure 8.

Effect of the CBR extract on the DSS-induced MAPK and NF-κB protein expression. (a) Immunoblotting analysis of phosphorylated MAPKs. Representative colon tissue lysates from normal mice administered with drinking water (control), mice administered with DSS-induced colitis (DSS), and mice coadministered with DSS and CBR extract (DSS + CBR) (from left to right). Protein expression of MAPK and NF-κB was analyzed by western blot analysis and quantified by densitometric analysis. (b) The immunohistochemical analysis was used to monitor the protein expression of NF-κB. Scale bar = 100 μm. Original magnification: 100x and 200x (top and bottom panels, resp.). (c) Immunoblotting analysis of NF-κB.