Table 1.
Gene | Primer sequence (5′ to 3′) | Annealing Temp. | Size (bp) |
Reference |
---|---|---|---|---|
AFP | GCTTGGTGGTGGATGAAACA TCCTCTGTTATTTGTGGCTTTTG |
62 | 157 | [22] |
ALB | TGAGAAAACGCCAGTAAGTGAC TGCGAAATCATCCATAACAGC |
62 | 265 | [22] |
CYP3A4 | CCTTACAT TACACACCCTTTGGAAGT AGCTCAATGCATGTACAGAATCCCCGGTTA |
62 | 382 | [22] |
HNF4α | GCCTACCTCAAAGCCATCAT GACCCTCCCAGCAGCATCTC |
62 | 275 | [22] |
AAT | ACATTTACCCAAACTGTCCATT GCTTCAGTCCCTTTCTCGTC |
56 | 183 | [23] |
TTR | GGTGAATCCAAGTGTCCTCTGAT GTGACGACAGCCGTGGTGGAA |
61 | 352 | [24] |
G6P | GCTGGAGTCCTGTCAGGCATTGC TAGAGCTGAGGCGGAATGGGAG |
56 | 350 | [23] |
TAT | CCCCTGTGGGTCAGTGTT GTGCGACATAGGATGCTTTT |
56 | 345 | [23] |
GAPDH | AGCCACATCGCTCAGACACC GTACTCAGCGGCCAGCATCG |
60 | 302 | [25] |
ALB: albumin; AFP: alpha-fetoprotein; AAT: alpha-1-antitrypsin; CYP3A4: cytochrome P450 type 3A4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; G6P: glucose-6-phosphatase; HNF4α: hepatocyte nuclear factor 4α; TAT: tyrosine aminotransferase; TTR: transthyretin; Temp.: temperature; bp: base pair.