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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Curr Protoc Toxicol. 2013 May;0 12:Unit12.20. doi: 10.1002/0471140856.tx1220s56

Figure 1.

Figure 1

BrdU is incorporated into SVZ-derived aNSCs. Adult neural stem cells from the SVZ were pulsed with BrdU for 2 h and cells fixed and processed for immunostaining as described. A) Cells grown without growth factor stimulation. B) Cells grown in the presence of 100 ng/mL EGF for 18 h. Hoechst is used a s counterstain to identify all cell nuclei. Scale bar in A represents 50 μm and applies to both panels.