Table 1.
BSA or human lactotransferrin added at a 5:1, 1:5 and 1:1 ratios, respectively. Either BSA or human lactotransferrin or both are added to the murine ES cell lysates and the ratio for each situation is established. The appropriate fold change can be detected, as the ratio of these marker proteins is known. Shown are the identifications, mass of protein, peptide number, percent of protein coverage, fold change, and mass of peptide
| Accession | Name | Mass | Peptides | % Coverage | Fold change | Peptide |
|---|---|---|---|---|---|---|
| 5:1 | ||||||
| gi|640213 | Chain C, legume isolectin Ii (Lol Ii) complexed with lactotransferrin (N2 Fragment) | 17 509 | 1 | 5 | 5.0388 DOWN | 1047.984 |
| gi|16198357 | Lactotransferrin [Homo sapiens] | 78 279 | 1 | 1 | 5.0388 DOWN | 1047.984 |
| gi|16198359 | Lactotransferrin [Homo sapiens] | 78 295 | 1 | 1 | 5.0388 DOWN | 1047.984 |
| gi|18490850 | Lactotransferrin [Homo sapiens] | 78 278 | 1 | 1 | 5.0388 DOWN | 1047.984 |
| gi|54607120 | Lactotransferrin [Homo sapiens] | 78 132 | 1 | 1 | 5.0388 DOWN | 1047.984 |
| gi|640213 | Chain C, legume isolectin Ii (Lol Ii) complexed with lactotransferrin (N2 Fragment) | 17 509 | 1 | 5 | 5.0901 DOWN | 1047.501 |
| gi|16198357 | Lactotransferrin [Homo sapiens] | 78 279 | 1 | 1 | 5.0901 DOWN | 1047.501 |
| gi|16198359 | Lactotransferrin [Homo sapiens] | 78 295 | 1 | 1 | 5.0901 DOWN | 1047.501 |
| gi|18490850 | Lactotransferrin [Homo sapiens] | 78 278 | 1 | 1 | 5.0901 DOWN | 1047.501 |
| gi|20151212 | Chain B, crystal structure of a domain-opened mutant (R121d) of the human lactoferrin N-lobe refine | 36 849 | 1 | 2 | 5.0901 DOWN | 1047.501 |
| gi|54607120 | Lactotransferrin [Homo sapiens] | 78 132 | 1 | 1 | 5.0901 DOWN | 1047.501 |
| gi|162648 | Albumin [Bos taurus] | 69 248 | 1 | 1 | 5.9942 DOWN | 1163.598 |
| gi|229552 | Albumin | 66 088 | 1 | 1 | 5.9942 DOWN | 1163.598 |
| gi|30794280 | Albumin [Bos taurus] | 69 278 | 1 | 1 | 5.9942 DOWN | 1163.598 |
| 1:5 | ||||||
| gi|7428767 | Lactotransferrin [Homo sapiens] | 78 320 | 1 | 1 | 5.0235 UP | 1578.824 |
| gi|16198357 | Lactotransferrin [Homo sapiens] | 78 279 | 1 | 1 | 5.0235 UP | 1578.824 |
| gi|16198359 | Lactotransferrin [Homo sapiens] | 78 295 | 1 | 1 | 5.0235 UP | 1578.824 |
| gi|18490850 | Lactotransferrin [Homo sapiens] | 78 278 | 1 | 1 | 5.0235 UP | 1578.824 |
| gi|54607120 | Lactotransferrin [Homo sapiens] | 78 132 | 1 | 1 | 5.0235 UP | 1578.824 |
| 1:1 | ||||||
| gi|16198357 | Lactotransferrin [Homo sapiens] | 78 279 | 1 | 1 | 2.3144 DOWN | 1616.128 |
| gi|16198359 | Lactotransferrin [Homo sapiens] | 78 295 | 1 | 1 | 2.3144 DOWN | 1616.128 |
| gi|18490850 | Lactotransferrin [Homo sapiens] | 78 278 | 1 | 1 | 2.3144 DOWN | 1616.128 |
| gi|54607120 | Lactotransferrin [Homo sapiens] | 78 132 | 1 | 1 | 2.3144 DOWN | 1616.128 |